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DNA hybridization study using Y-specific probes in an XX-male

✍ Scribed by Dr. H. Fuse; S. Satomi; T. Kazama; T. Katayama; S. Nagabuchi; T. Tamura; Y. Nakahori; Y. Nakagome


Book ID
115137221
Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
547 KB
Volume
23
Category
Article
ISSN
0303-4569

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Detection of Y-bearing porcine spermatoz
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This study was carried out to determine whether Y-bearing porcine spermatozoa could be detected by in situ hybridization using a digoxigenin (Dig)-labelled DNA probe specific to the Y chromosome produced by polymerase chain reaction (PCR). A conventional PCR (with Dig-dUTP) was performed using a set