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DNA Handles for Single Molecule Experiments

✍ Scribed by Martin Hegner


Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
297 KB
Volume
1
Category
Article
ISSN
1438-5163

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We used a bead displacement sensor to determine the enzymatic shortening of individual molecules of unstained -DNA attached to optically trapped beads. The setup has been described previously (Dapprich and Nicklaus: Bioimaging 6:25-32, 1998) and works by observing the change in position of a trapped