DNA “fingerprints” applied to paternity analysis in apples (Malus x domestica)

✍ Scribed by H. Nybom; B. A. Schaal

• Book ID: 104671094
• Publisher: Springer
• Year: 1990
• Tongue: English
• Weight: 862 KB
• Volume: 79
• Category: Article
• ISSN: 0040-5752
• DOI: 10.1007/bf00224242

✦ Synopsis

Analysis of minisatellite DNA sequences, yielding so called DNA "fingerprints", has proven useful in paternity analysis for several different organisms. Here 64 apple seedlings, grown from seeds collected in an orchard with three cultivars, were analyzed using the M13 "fingerprint" probe. Paternity could be determined for 56 of the seedlings, 2 of which were derived through selfing. The analysis was facilitated by the occurrence of a multiallelic locus. The five different fragments determined by this locus migrated to similar positions, whether digesting the DNA with restriction enzymes TaqI or RsaI.