DNA fingerprinting of conjugative plasmids incompatible with R6K (IncX)

✍ Scribed by Dr. Rita Prager; Erhard Tietze; Helmut Tschäpe

• Book ID: 102390961
• Publisher: John Wiley and Sons
• Year: 1988
• Tongue: English
• Weight: 222 KB
• Volume: 28
• Category: Article
• ISSN: 0233-111X
• DOI: 10.1002/jobm.3620280610

✦ Synopsis

14 conjugative plasmids originated from different bacterial hosts and geographical sources were identified as members ofthe incompatibility group IncX with R6K as the reference plasmid. In spite oftheir close genetic relatedness (transfer and incompatibility properties), their molecular sizes ranged between 39 and 77 kb and DNA fingerprinting with endonucleases such as EcoRI, EcoRV, BmH1, Bgn, BgflI, Psi1 and Sun revealed a great variety of fragment patterns.