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DNA fingerprinting in rice using oligonucleotide probes specific for simple repetitive DNA sequences

✍ Scribed by W. Ramakishana; M. D. Lagu; V. S. Gupta; P. K. Ranjekar

Publisher
Springer
Year
1994
Tongue
English
Weight
875 KB
Volume
88-88
Category
Article
ISSN
0040-5752

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✦ Synopsis

In this report we describe the use of five oligonucleotide probes, namely (GATA)4, (GACA)4, (GGAT)4, (GAA)6 and (CAC)5, to reveal highly polymorphic DNA regions in rice. With each of the oligonucleotide probes, the level of polymorphism was high enough to distinguish several rice genotypes. Moreover, individual plants of one cultivar showed the same cultivar-specific DNA fingerprint. The multilocus fingerprint patterns were somatically stable. Our study demonstrates that microsatellite-derived DNA fingerprints are ideally suited for the identification of rice genotypes. As the majority of the probes detected a high level of polymorphism, they can be very useful in monitoring and aiding gene introgression from wild rice into cultivars.

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