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DNA diagnosis of hydatidiform mole using the polymerase chain reaction

✍ Scribed by Ryuichi Fukuyama; Mizuho Takata; Jun Kudoh; Kosuke Sakai; Shozo Tamura; Nobuyoshi Shimizu

Publisher: Springer
Year: 1991
Tongue: English
Weight: 371 KB
Volume: 87
Category: Article
ISSN: 0340-6717
DOI: 10.1007/bf00204186

No coin nor oath required. For personal study only.

✦ Synopsis

We have used the polymerase chain reaction (PCR) technique for the diagnosis of hydatidiform mole, a trophoblastic disease. For this, we targeted the hypervariable 3' flanking region of the APOB gene (APOB/ VNTR) because of its high heterozygosity index (0.61) in the Japanese population. We examined seven clinical cases which were tentatively diagnosed as hydatidiform moles. Five of these revealed DNA segments unique to the paternal APOB allele, allowing us to diagnose a complete mole. The PCR technique for targeting the APOB/VNTR appears useful for early diagnosis of hydatidiform mole.

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