DNA degradation and reduced recombination following UV irradiation during meiosis in yeast (Saccharomyces cerevisiae)
✍ Scribed by Salts, Yehiam ;Simchen, Giora ;Pi�on, Ramon
- Publisher
- Springer
- Year
- 1976
- Tongue
- English
- Weight
- 460 KB
- Volume
- 146
- Category
- Article
- ISSN
- 0026-8925
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✦ Synopsis
Irradiation of meiotic yeast cells with moderate doses of ultraviolet irradiation (1,600 erg/mm2) leads to the arrest of premeiotic DNA synthesis, massive (5-40%) DNA degradation, and a 40-50% loss of cell viability. In contrast, such doses of UV irradiation had a minor effect on viability (15-20% loss) of logarithmically growing cells, and no comparable DNA degradation was observed in irradiated synchronized vegetative cells. Meiotic recombination is also affected by UV irradiation. When administered at a stage comparable to meiotic prophase, low doses of irradiation result in a reduction in recombination frequency without significantly affecting cell viability.