DNA Damage Stimulates Human Cell Transformation by Integrative But Not Episomal Epstein-Barr Virus-Derived Plasmid
✍ Scribed by Jean-Michel H. Vos; Eliane L. Wauthier; Philip C. Hanawalt
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 660 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0899-1987
No coin nor oath required. For personal study only.
✦ Synopsis
Previous work has demonstrated that ultraviolet (UV) irradiation of SV40-based plasmids can strikingly enhance the frequency of stable transformation of human cells. In this study we compared the effect o f UV-induced DNA damage on transformation mediated by integrative versus autonomously replicating plasmids derived from human Epstein-Barr virus (EBV). We report that transfection of human fibroblasts with UV-irradiated integrative EBV-based plasmid results in enhanced transformation. However, transfection of UV-damaged episomal EBVbased constructs into the same human cell line does not enhance transformation; in fact, the extrachromo-soma1 status of the plasmid is maintained irrespective o f the UV dose t o the plasmid. We conclude that enhanced transformation of human cells by damaged DNA requires its chromosomal integration.