DNA damage in nasal respiratory epithelium from children exposed to urban pollution

✍ Scribed by Lilian Calderón-Garcidueñas; Norma Osnaya; Antonio Rodriguez-Alcaraz; Anna Villarreal-Calderón

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 816 KB
• Volume: 30
• Category: Article
• ISSN: 0893-6692
• DOI: 10.1002/(sici)1098-2280(1997)30:1<11::aid-em3>3.0.co;2-f

✦ Synopsis

T he nas al c avit y is t he m os t co m m on por t al of en t ry E leven-and t we lve-year-olds had t he m os t DNA t o t he hum an body and a we ll-kn own t arget s it e dam age, and m or e t han 30% of ch ildren aged 9for a wide range of air polluta nt s and c hem ic ally 12 exhibit ed pat c hy are as of s quam ous m et aplas ia induc ed t oxicit y and carc ino genic it y. DNA si ngle-over high-flo w nas al regions . T hes e are as had the s t rand break s (S SB ) c an be use d as a biom ark er of great es t num bers of dam aged DNA c ells ( P °oxidant exposu re and as an ind ic at or of t he c arc ino-0.001) and a large num ber of DNA t ails ú 80 mm genic it y and mu t agenicit y of a s ubs ta nc e. We ex-( P õ 0.001) when compared to the contralateral am ined th e ut ilit y of us ing th e alk aline s ingle c ell gel m ac ros co pic ally norma l s it e in t he sa m e c hild. T he elec t rophores is as s ay (SCGE) for m eas uring DNA younges t c hildren wit h s ignifi c ant ly les s outd oor exdam age in c hildren's nas al epit helium expose d to pos ure dis played pat ch y areas of goblet c ell hyperair pollu t ants . We s t ud ied 148 c hildren, ages 6-plas ia and had t he leas t DNA dam age. Th es e fin d-12, inc lu ding 19 c ont ro l c hildren fro m a low pol-ings s ugges t th at S CGE c an be us ed t o mo nit or lut ed P acific por t and 129 c hildren from S out hwe s t DNA dam age in ch ildren's nasa l epit he lium and, Met ropolit an Mexic o Cit y, an urban pollut ed area furt her, t he ident ific at ion of DNA dama ge in nas al wit h high ozone c onc ent rat ions year-round. T hree prolifer at ive epit helium co uld be re garded as a s ens et s of tw o nasa l biopsi es wer e t ak en in a 3-m on t h t inel les ion, m os t like ly due t o s ever e and s us t ained period. All expos ed c hildren had upper res pirat ory