DNA damage and toxicogenomic analyses of hydrogen sulfide in human intestinal epithelial FHs 74 Int cells

✍ Scribed by Matias S. Attene-Ramos; Gerardo M. Nava; Mark G. Muellner; Elizabeth D. Wagner; Michael J. Plewa; H. Rex Gaskins

• Book ID: 102833231
• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 516 KB
• Volume: 51
• Category: Article
• ISSN: 0893-6692
• DOI: 10.1002/em.20546

✦ Synopsis

Hydrogen sulfide (H 2 S), a metabolic end product of sulfate-reducing bacteria, represents a genotoxic insult to the colonic epithelium, which may also be linked with chronic disorders such as ulcerative colitis and colorectal cancer. This study defined the early (30 min) and late (4 hr) response of nontransformed human intestinal epithelial cells (FHs 74 Int) to H 2 S. The genotoxicity of H 2 S was measured using the single-cell gel electrophoresis (comet) assay. Changes in gene expression were analyzed after exposure to a genotoxic, but not cytotoxic, concentration of H 2 S (500 lM H 2 S) using pathway-specific quantitative RT-PCR gene arrays. H 2 S was genotoxic in a concentration range from 250 to 2,000 lM, which is similar to concentrations found in the large intes-tine. Significant changes in gene expression were predominantly observed at 4 hr, with the greatest responses by PTGS2 (COX-2; 7.92-fold upregulated) and WNT2 (7.08-fold downregulated). COX-2 was the only gene upregulated at both 30 min and 4 hr. Overall, the study demonstrates that H 2 S modulates the expression of genes involved in cell-cycle progression and triggers both inflammatory and DNA repair responses. This study confirms the genotoxic properties of H 2 S in nontransformed human intestinal epithelial cells and identifies functional pathways by which this bacterial metabolite may perturb cellular homeostasis and contribute to the onset of chronic intestinal disorders.