DNA Damage and Repair

✍ Scribed by R. B. Setlow (auth.), Amleto Castellani (eds.)

• Publisher: Springer US
• Year: 1989
• Tongue: English
• Leaves: 376
• Edition: 1
• Category: Library

✦ Synopsis

The First International Congress on DNA Damage and Repair was held in Rome, Italy, July 12-17, 1987. It was organized by the Italian Com­ mission for Nuclear Alternative Energy Sources. The subject of DNA damage and repair involves almost all the fields ofbidogical sciences. Some of the more prominent ones include carcino­ genesis, photobiology, radiation biology, aging, enzymology, genetics, and molecular biology. These individual fields have their own interna­ tional meetings and although the meetings often have sessions devoted to DNA repair, they do not bring together a wide diversity of international workers in the field to exchange ideas. The purpose of the Congress was to facilitate such an exchange among scientists representing many fields of endeavor and many countries. The 37 manuscripts in this volume, presented by the invited spea­ kers during the four and half days of the Congress, encompass the field of DNA damage and repair. They cover biological systems ranging from mo­ lecules to humans and deal with damages and repair after treatment of cells with various types of radiations, chemicals, and exogenous and en­ dogenous oxidative damages. The Congress and its Proceedings are dedicated to two international leaders in the field of DNA damage and repair, Alexander Hollaender of the United States and Adriano Buzzati Traverso of Italy. Hollaender, who died in December 1986, was one of the first investigators to recognize the damage to DNA was important in cell killing and mutagenesis. His early work indicated that cells could recover from radiation injury.

✦ Table of Contents

Front Matter....Pages i-xi
Twenty Five Years of DNA Repair....Pages 1-9
Macromolecular Physiology of the Escherichia coli UVR Proteins....Pages 11-15
Toward an Understanding of Nucleotide Excision Repair in Yeast: A Summary of Recent Progress....Pages 17-25
Isolation and Characterization of Genes Involved in Mammalian Excision Repair....Pages 27-36
Selective DNA Repair in the Transcribed Strands of Active Genes in Mammalian Cells....Pages 37-42
Chromosomal Mapping of Human DNA Repair Genes....Pages 43-49
Inhibiting DNA Repair — Models, Manipulations and Misconceptions....Pages 51-60
The Association of Xeroderma Pigmentosum with Trichothiodystrophy: A Clue to a Better Understanding of XP-D?....Pages 61-72
In Vitro Correction of Cells from Patients with Mutagen Hypersensitivity....Pages 73-82
DNA Alkylation Adducts in Human Cells Attributable to Exposure to Alkylating Agents....Pages 83-86
DNA Repair of Alkylation Damage and Its Relevance to Mutation Fixation in Mammalian Cells....Pages 87-95
Mechanisms of Alkylating Agent Induced Cytotoxicity in E.coli and Mammalian Cells....Pages 97-106
The Response of Human Cells to In Vivo Methylation Damage....Pages 107-123
Repair of Secondary Lesions Arising in DNA after Treatment with Alkylating Agents....Pages 125-133
Inducible Repair of Alkylated Bases in Mammalian Cells....Pages 135-141
DNA Strand Bias During O 6 -Methylguanine Mutagenesis....Pages 143-150
Formation and Enzymatic Repair of Specific Reaction Products of Alkylating N-Nitroso Carcinogens in Cellular DNA: Relevance to Malignant Transformation....Pages 151-156
DNA Damage and Repair in Human Skin In Situ ....Pages 157-168
DNA Repair and Mutagenesis Induced by Dimer and Non-Dimer Photoproducts Measured with Plasmid Vectors in Xeroderma Pigmentosum Cells....Pages 169-181
Molecular Mechanisms of Mutagenesis Induced by Chemical Carcinogens and CIS-DDP....Pages 183-195
Maintenance of 5-Methylcytosine in Mammalian Cells by Specific Correction of G/T Mispairs in Favor of Guanine....Pages 197-203
A Shuttle Vector for Investigating the Molecular Mechanisms of Mutagenesis in Mammalian Cells in Culture....Pages 205-210
Mammalian DNA Ligase : Studies on the Structure and Function during DNA Replication and Repair....Pages 211-222
Antineoplastic Agents Inhibitor of Topoisomerase II....Pages 223-241
DNAase I Hypersensitive Sites of the c-Ha- ras -1 Proto-Oncogene as Targets for Rapid Benzo[ a ]pyrene Binding and Repair....Pages 243-249
Visualization and Computer-Assisted Quantification of DNA Modifications in Individual Cells....Pages 251-262
A Short Review of Methods for Detecting DNA Fragmentation and Associated Phenomena....Pages 263-270
DNA Unwinding: Common Modification Induced by “Bulky Adducts” to DNA Structure....Pages 271-279
Mechanisms of Hydrogen Peroxide Cytotoxicity in Mammalian and Bacterial Cells....Pages 281-289
Cancer, Aging, and Endogenous DNA Damage....Pages 291-298
Processing of Thymine Glycols, Urea Residues and AP Sites in Escherichia Coli ....Pages 299-304
Low Dose Radiation Effects in Human Cells....Pages 305-311
Multiple Roles for Poly(ADP-Ribose) Synthesis in Repair of DNA Damage and Malignant Transformation....Pages 313-328
The Induction and Repair of Double-Strand DNA Breaks in Mammalian Cells as Detected by Neutral Elution....Pages 329-344
Damage, Repair, and DNA Synthesis in Radiation Lethality of Mammalian Cells....Pages 345-358
X-rays Induced Double Strand Breaks: Damage Distribution and Measurement....Pages 359-372
Killing, Stress Responses and Mutagenesis Induced in E. Coli by Hydrogen Peroxide....Pages 373-376
Back Matter....Pages 377-383

✦ Subjects

Biochemistry, general