DNA constraints on transcription activation in Vitro
โ Scribed by Eric D Ross; Anne M Keating; L.James Maher III
- Book ID
- 115630064
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 429 KB
- Volume
- 297
- Category
- Article
- ISSN
- 0022-2836
No coin nor oath required. For personal study only.
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E coli RNA polymerase holoenzyme is able to recognize transcription initiation sites on Adenovirus 2 DNA that are functionally indistinguishable from promoters for the enzyme on phage DNAs. The complexes formed between the polymerase and the DNA at these sites can exist in two states-either as I (in
We estimate that E. coli RNA polymerase is able to form stable, rifampicin-resistant, pre-intiation complexes with Adenovirus 2 DNA at three to six binding sites. The number of RNA chains initiated from such complexes has been determined form the incorporation of gamma-32P-ATP and -GTP at two rifamp