DNA blotting analysis of human retroviruses in cerebrospinal fluid of spastic paraparesis patients: The viruses are identical to human T-cell leukemia virus type-1 (HTLV-1)
✍ Scribed by Jun Imamura; Atsumi Tsujimoto; Yoshihiro Ohta; Shozo Hirose; Kunitada Shimotohno; Masanao Miwa; Isao Miyoshi
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- French
- Weight
- 741 KB
- Volume
- 42
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
The structure of the integrated provirus in cell lines established from the cerebrospinal fluid (CSF) of patients with human T-cell leukemia virus type-I (HTLV-I)-associated myelopathy (HAM) was analyzed. The digestion patterns with 3 restriction endonucleases, Sac I, Eco RI and Pst I, of the proviruses integrated in T-lymphoid cell lines derived from the CSF of 4 HAM patients were similar to those of HTLV-I from adult T-cell leukemia (ATL) patients. Integrated proviruses in one of these cell lines derived from the CSF were further analyzed in detail with 2 more restriction enzymes, BarnHl and Sma 1. The results indicate that the retrovirus found in lymphoc tes of the CSF from atients with HAM are very similar, not identical, to H'TPLV-I found in the leukemic lymphocytes of ATL patients.