DNA binding properties of dioxin receptors in wild-type and mutant mouse hepatoma cells
β Scribed by Cuthill, Scott; Poellinger, Lorenz
- Book ID
- 127357588
- Publisher
- American Chemical Society
- Year
- 1988
- Tongue
- English
- Weight
- 571 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0006-2960
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π SIMILAR VOLUMES
We analyzed the function of a DNA domain located upstream of the cytochrome P4501A1 gene in wild-type (Hepa lclc7) mouse hepatoma cells and in high-activityvariant (HAV) cells that overtranscribe the gene in response to the inducer 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Transfection experiments
T4-infected cells, plasmolysed 15 rnin after infection, incorporate low concentrations (< 20 taM) of deoxythymidine (TdR) into DNA at a significantly greater rate than dTMP, dTTP or thymine. At higher concentrations (> 40 gM), dTMP incorporation rate is high, approaching that of TdR at 200 laM. TdR