DNA-Based Gels for Oral Delivery of Probiotic Bacteria

✍ Scribed by Benjamas Jonganurakkun; Yoshinobu Nodasaka; Nobuo Sakairi; Norio Nishi

• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: English
• Weight: 185 KB
• Volume: 6
• Category: Article
• ISSN: 1616-5187
• DOI: 10.1002/mabi.200500199

✦ Synopsis

Abstract

Summary: A single‐stranded DNA, readily extracted from industrial discarded salmon milt, was used to prepare hydrogels and complex gels by cross‐linking with gelatin and κ‐carrageenan, for the oral delivery of probiotic bacteria. The complex gels showed a higher protective capability over the hydrogels for approximately one log scale. However, the hydrogels were more stable during storage at 4 °C. The Lactobacillus and Lactococcus due to protection of the hydrogels could better tolerate to acid than the Bifidobacterium. Furthermore, food‐graded hydrogels were prepared and optimized to a similar protective capability for future applications.

SEM of bacteria fixed in DNA‐based hydrogel and DNA‐based complex gel.

magnified imageSEM of bacteria fixed in DNA‐based hydrogel and DNA‐based complex gel.