DNA Base Composition of Rickettsia tsutsugamushi Determined by Reversed-Phase High-Performance Liquid Chromatography
โ Scribed by KUMURA, K.; MINAMISHIMA, Y.; YAMAMOTO, S.; OHASHI, N.; TAMURA, A.
- Book ID
- 121288341
- Publisher
- Society for General Microbiology
- Year
- 1991
- Tongue
- English
- Weight
- 401 KB
- Volume
- 41
- Category
- Article
- ISSN
- 0020-7713
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๐ SIMILAR VOLUMES
DNA base composition was determined by reversed-phase high-performance liquid chromatography (HPLC). DNA was hydrolysed into nucleosides with nuclease P1 and bacterial alkaline phosphatase. The mixture of nucleosides was applied to HPLC without any further purification. One determination by chromato
A method to separate the four major bases (cytosine, guanine, thymine and adenine) and the two minor modified bases (5-methylcytosine and 6N-methyladenine) in DNA has been developed. For optimal separation, several different buffer systems are available for isocratic elution. The 12 5-methylcytosine