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DNA attachment to nanocrystalline diamond films

✍ Scribed by Wenmackers, S. ;Christiaens, P. ;Daenen, M. ;Haenen, K. ;Nesládek, M. ;van deVen, M. ;Vermeeren, V. ;Michiels, L. ;Ameloot, M. ;Wagner, P.

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
191 KB
Volume
202
Category
Article
ISSN
0031-8965

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✦ Synopsis

Abstract

A biochemical method to immobilize DNA on synthetic diamond for biosensor applications is developed. Nanocrystalline diamond is grown using microwave plasma‐enhanced chemical vapour deposition. On the hydrogen‐terminated surface 10‐undecenoic acid is tethered photochemically under 254 nm illumination, followed by 1‐ethyl‐3‐[3‐dimethylaminopropyl]carbodiimide crosslinker‐mediated attachment of amino modified DNA. The attachment is functionally confirmed by comparison of supernatant fluorescence and gel electrophoresis. The linking procedure allowed for 35 denaturation and rehybridisation steps. (© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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