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DNA affinity binding studies using a fluorescent dye displacement technique: the dichotomy of the binding site

✍ Scribed by Caitriona B. Spillane; Jayden A. Smith; Joy L. Morgan; F. Richard Keene


Book ID
106232081
Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
256 KB
Volume
12
Category
Article
ISSN
1432-1327

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Quantitative affinity chromatography: In
✍ Douglas A. Bergman; Donald J. Winzor πŸ“‚ Article πŸ“… 1986 πŸ› Elsevier Science 🌐 English βš– 627 KB

The potential of affinity chromatography for the characterization of strong solute-ligand interactions is explored by studying the NADHdependent elution of rabbit muscle lactate dehydrogenase from a column of trinitrophenyl-Sepharose in 0.067 M phosphate, pH 7.2. An interesting development is the si