DNA adducts in urothelial cells: Relationship with biomarkers of exposure to arylamines and polycyclic aromatic hydrocarbons from tobacco smoke
✍ Scribed by Paolo Vineis; Glenn Talaska; Christian Malaveille; Helmut Bartsch; Tiziana Martone; Pornchai Sithisarankul; Paul Strickland
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- French
- Weight
- 320 KB
- Volume
- 65
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Markers of exposure to polycyclic aromatic hydrocarbons (urinary I -hydroxypyrene-glucuronide) and aromatic arnines (4-aminobiphenyl-hemoglobin adducts), as well as urinary mutagenicity, were measured in 47 healthy smokers and 50 nonsmokers. DNA adducts were determined by P32-postlabeling in the exfoliated bladder cells of 39 healthy subjects. Both I -hydroxypyrene-glucuronide (I -0HPG) and 4-aminobiphenyl adducts (4-ABP-Hb) were associated with smoking habits, but only 4-ABP-Hb adducts were associated with consumption of black, air-cured tobacco. The levels of 2 DNA adducb (numbers 2 and 4) in urothelial cells were clearly associated with 4-ABP-Hb adducts, in all subjects and in smokers. Levels of one of these DNA adducts (number 2) were also associated with I -hydroxypyrene-glucuronide in urines, but in smokers the association was not statistically significant. Overall, these observations constitute further evidence of a role of arylamines in tobaccoinduced bladder cancer.