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DLA-DRB1 histocompatibility genotyping using RT-nested PCR and cycle sequencing

✍ Scribed by George M Happ; Elizabeth Aquilla; Monika Martick; Christine Yuncker; Janet Wojciechowski; Leslie Fox


Book ID
117336916
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
286 KB
Volume
69
Category
Article
ISSN
0165-2427

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Three nested RT-PCR assays were developed to permit sensitive typing of enteroviruses directly from clinical samples. These assays amplified short fragments from different genomic regions codifying for three proteins: VP2, VP1, and RNA polymerase. Given that enteroviruses have a high rate of degener