Diversity of T cell receptor α and β chain genes expressed by human T cells specific for similar myelin basic protein peptide/major histocompatibility complexes
✍ Scribed by Gerhard Giegerich; Martin Pette; Edgar Meinl; Jörg T. Epplen; Hartmut Wekerle; Ari Hinkkanen
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 693 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0014-2980
No coin nor oath required. For personal study only.
✦ Synopsis
Diversity of T cell receptor a and p chain genes expressed by human T cells specific for similar myelin basic protein peptide/major histocompatibility complexes* Tcell receptor (TcR) a and p nucleotide sequences involved in the human autoreactivity to myelin basic protein (MBP) were studied by screening cDNA libraries derived from 11 independent T lymphocyte clones (TCC) established from multiple sclerosis patients and healthy donors. The TCC with defined MBP peptide specificity and HLA-DR restriction expressed multiple TcR. Even TCC recognizing the same human MBP peptide [amino acids (aa) 139-1533 in identical or very similar HLA-DR context expressed diverse TcR. Two TCC which recognized peptide aa 139-153 equally well in the context of both HLA-DR2a and -DR1 molecules used distinct TcR a but identical fI chains.
The knowledge of TcR and TcR a chain sequences of human MBP-specific Tcells will allow studies correlating structure and function of TcR and their targets in MBP autoreactivity. This may have an impact on the development of immunotherapies in multiple sclerosis.