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Diversity inParacoccidioides brasiliensis.The PbGP43gene as a genetic marker

✍ Scribed by Rosana Puccia; Juan G. McEwen; Patrícia S. Cisalpino


Book ID
106475686
Publisher
Springer Netherlands
Year
2008
Tongue
English
Weight
290 KB
Volume
165
Category
Article
ISSN
0301-486X

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A system is described for gene disruption and replacement in Schizosaccharomyces pombe based on the homologous selectable marker, ura4, the structural gene for orotidine-5'-phosphate decarboxylase. The presence of a single copy of the wild-type gene can rescue a ura4 auxotrophic mutant. Furthermore,