Divergent selection for in vitro developmental capacity of preimplantation mouse embryos

✍ Scribed by D. Pomp; E. S. Critser; J. J. Rutledge

• Publisher: Springer
• Year: 1988
• Tongue: English
• Weight: 604 KB
• Volume: 76
• Category: Article
• ISSN: 0040-5752
• DOI: 10.1007/bf00265352

✦ Synopsis

Replicated divergent selection was conducted for two generations in ICR mice for in vitro developmental capacity (IVDC; percentage of fertilized one-cell zygotes developing to blastocysts in vitro per female donor). Realized heritabilities based on high and low selection were 0.03±0.08 and -0.11±0.09 in replicate 1, and 0.10±0.11 and 0.08±0.10 in replicate 2. No differences were detected between selection lines (P>0.2) or replicates (P>0.1). Estimate of heritability in the base population based on 332 daughter-dam pairs was 0.14±0.18. These results indicate that additive genetic variance contributes little to the phenotypic variance in this trait. Considerable phenotypic variation in IVDC was observed (mean=49.3; SD=31.0), with a range of IVDC from 0%-100%. Utilization of donor female as a blocking factor is suggested for designs of experiments with preimplantation embryos to increase precision and power of statistical analyses.