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Dithio-phospholipids for oriented immobilization of proteins to gold surfaces

✍ Scribed by Gerald Kada; Christian K Riener; Hermann J Gruber

Book ID
104230335
Publisher
Elsevier Science
Year
2001
Tongue
French
Weight
131 KB
Volume
42
Category
Article
ISSN
0040-4039

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✦ Synopsis

We report the syntheses of phospholipids containing a dithio-group on their hydrophobic end and a choline or a biotin-group on their hydrophilic end. As demonstrated by surface plasmon resonance, a monolayer of these dithiolipids on gold affords specific binding of streptavidin and of biotinylated molecules in further steps.

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Immobilization of proteins to a carboxymethyldextran-modified gold surface for biospecific interaction analysis in surface plasmon resonance sensors
✍ Bo Johnsson; Stefan LΓΆfΓ₯s; Gabrielle Lindquist πŸ“‚ Article πŸ“… 1991 πŸ› Elsevier Science 🌐 English βš– 1006 KB

A method for fast and simple covalent immobilization of proteins to a carboxymethyldextran-modified gold surface intended for surface plasmon resonance sensors is described. The method utilizes the formation of N-hydroxysuccinimide esters from a fraction of the carboxyl groups of the carboxymethylde