Probing the disulfide folding pathway of
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Steven J. Milner; John A. Carver; F. John Ballard; Geoffrey L. Francis
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Article
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1999
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John Wiley and Sons
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English
⚖ 241 KB
👁 1 views
The crucial step of folding of recombinant proteins presents serious challenges to obtaining the native structure. This problem is exemplified by insulin-like growth factor (IGF)-I which when refolded in vitro produces the native three-disulfide structure, an alternative structure with mispaired dis