Distribution patterns of rabbit embryos during preimplantation stage
β Scribed by Yoshio Tsutsumi; E. S. E. Hafez
- Publisher
- John Wiley and Sons
- Year
- 1974
- Tongue
- English
- Weight
- 843 KB
- Volume
- 144
- Category
- Article
- ISSN
- 0362-2525
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β¦ Synopsis
Abstract
The pattern of transport and distribution of rabbit embryos in the oviduct and uterus was studied 15 to 168 hours post coitum (p. c.). The reproductive tract was frozen in liquid nitrogen, thawed, and cleared in benzylβbenzoate solution using Orsini's technique. The location of the eggs and the ampullaryβisthmic junction were identified using transmitted light from a dissecting microscope. Accumulation of the eggs in the oviduct occured in two phases. In the first phase the eggs were retained above the ampullaryisthmic junction, 3β12 hours after ovulation. In the second phase, the eggs were retained 36β60 hours after ovulation, above the uterotubal junction (at a distance approximately 12 % of the oviductal length). The rate of transport of individual eggs in the oviduct, and the time of the entry of eggs into the uterus were variable. Au 78 hours p. c. most blastocysts occupied the proximal half of the uterine horn, although some appeared very close to the internal os of the cervix. Spacing of blastocysts in the uterus, 114 to 120 hours p. c., involved movement of blastocysts away from the cervix. Unfertilized eggs remained in the uterus, along with developing blastocysts 168 hours p. c. Few eggs were retained in the oviduct at 108 and 115 hours p. c.
π SIMILAR VOLUMES
## Abstract Mammals, like all multicellular organisms, develop from a single cellβthe totipotent zygote. During preimplantation development and subsequent development in utero, over 200 distinct cell types are established and integrated into the organ systems and tissues of the developing organism.