Distribution of35S-Sulfate within the transseptal ligment of the mouse

✍ Scribed by Johnson, Roger B.

• Book ID: 102743225
• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 811 KB
• Volume: 223
• Category: Article
• ISSN: 0003-276X
• DOI: 10.1002/ar.1092230203

✦ Synopsis

The distribution of 35S-sulfate-labeled macromolecules was examined within three regions of the transseptal ligament: the 1) mesial, 2) middle and 3) distal thirds. Swiss mice, 6 weeks of age, were injected with 35S-sulfate and killed after 1, 6, and 12 hours and 1, 2, 3, 4, 5 , and 7 days. Silver grains and cell nuclei were counted on autoradiographs which had been counterstained by the Van Gieson method, and mean counts were analyzed statistically. Analysis of variance revealed no significant differences in mean number of cell nuclei between regions throughout the course of the experiment. 35S-sulfate was rapidly incorporated into the transseptal ligament macromolecules. Grain counts were highest 6 hours after injections: counts were highest over the middle and lowest over the mesial thirds of the ligament. The rate of grain removal was significantly higher in the middle third compared to the mesial or distal thirds (P< 0.001) and was significantly lower in the mesial third compared to the middle or distal thirds (P< 0.001). The half-life of