𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Distribution of the inducer of differentiation bis-acetyl-diaminopentane in murine erythroleukemia cells

✍ Scribed by Asterios S. Tsiftsoglou; Kuldeep K. Bhargava; Lana S. Rittmann; Alan C. Sartorelli

Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
489 KB
Volume
106
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Exposure of murine leukemia cells in culture to bis‐acetyl‐diaminopentane (BADP) caused erythroid maturation as measured by the accumulation of hemoglobin in treated cells. The appearance of differentiated cells in cultures exposed to BADP occurred 18 to 20 hours earlier than in those treated with dimethylsulfoxide (DMSO), a standard inducer of differentiation in this system. Studies with [^3^H]BADP indicated the occurrence of relatively rapid association of the inducer with cells, and subsequent linear accumulation. Fractionation of cellular components and measurement of radioactivity from BADP therein demonstrated that this agent preferentially associates with a fraction enriched for plasma membrane. In addition, [^3^H]BADP was capable of binding to the plasma membrane‐enriched fraction isolated from murine erythroleukemia cells as measured by gel filtration. These findings support the concept that interaction of inducers of murine erythroleukemia differentiation such as BADP with components of the surface membrane may be important in the cascade of events that lead to the erythroid maturation of these leukemic cells.

📜 SIMILAR VOLUMES

Induction of erythroid differentiation i
Induction of erythroid differentiation in murine erythroleukemia cells by N-substituted polymethylene diamides
✍ Toyoharu Hozumi; Junko Nomura; Minoru Ishizawa 📂 Article 📅 1979 🏛 John Wiley and Sons 🌐 French ⚖ 391 KB

## Abstract Various N‐substituted polymethylene diamides were synthesized and tested for their potency to induce erythroid differentiation in murine erythro‐leukemia cells. N,N,N′,N′‐tetramethyl‐1.6‐hexane‐dicarboxamide (IIc) was the most potent inducer among 15 compounds tested. The effectiveness

The effect of differentiation inducers o
The effect of differentiation inducers on the integrin expression of K562 erythroleukemia cells.
✍ Maarit Järvinen; Jari Ylänne; Ismo Virtanen 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 465 KB

## Abstract We studied the effects of differentiation‐inducers on the integrin profile and adhesive properties of K562 leukemia cells. The fibronectin (Fn) receptor integrin, α~5~β~1~, was the only integrin expressed in suspension cultured K562 cells. When the cells were exposed to 12‐O‐tetradecano

CLONING, SEQUENCING AND EXPRESSION OF A
CLONING, SEQUENCING AND EXPRESSION OF A cDNA ENCODING THE MOUSE L35a RIBOSOMAL PROTEIN DURING DIFFERENTIATION OF MURINE ERYTHROLEUKEMIA (MEL) CELLS
✍ I.S. Pappas; I.S. Vizirianakis; A.S. Tsiftsoglou 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 407 KB

In a previous study we reported that ribosomal protein S5 gene is suppressed in differentiating and not in proliferating or apoptotic murine erythroleukaemia (MEL) cells (Vizirianakis et al., 1999). We wish to report here the isolation, characterisation and expression of the full length cDNA for ano

Constitutive muscarinic receptors are in
Constitutive muscarinic receptors are involved in the growth and differentiation of friend erythroleukemia cells
✍ Cristina Cellai; Rosanna Matucci; Alessandro M. Vannucchi; Francesco Paoletti 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 199 KB

Binding experiments with the specific muscarinic ligand [ 3 H]N-methylscopolamine ( 3 H-NMS) have shown the presence of constitutive muscarinic acetylcholine receptors (mAChR) on Friend murine erythroleukemia cells (MELC). Competition experiments with a panel of specific antagonists indicated that t

Down-regulation of c- myc and bcl-2 gene
Down-regulation of c- myc and bcl-2 gene expression in PU.1-induced apoptosis in murine erythroleukemia cells
✍ Fumiko Kihara-Negishi; Toshiyuki Yamada; Yoshiko Kubota; Nobuo Kondoh; Hitomi Ya 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 French ⚖ 223 KB 👁 3 views

We found that over-expression of PU.1, a member of the ets family of transcription factors, induces apoptotic cell death along with differentiation of DMSO stimulation in murine erythroleukemia (MEL) cells. To elucidate the molecular mechanisms of apoptosis, cell-cycle distribution and expression of

Distribution and local differentiation o
Distribution and local differentiation of mast cells in the parenchyma of the forebrain
✍ Zhuang, X.; Silverman, A.-J.; Silver, R. 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 641 KB

Mast cells are found in the brain of many species. Although a considerable body of information is available concerning the development and differentiation of peripheral mast cells, little is known about brain mast cells. In the present study, the ontogeny of mast cells in the dove brain was followed