Distribution of SH-SY5Y human neuroblastoma cells in the cell cycle following exposure to organophosphorus compounds

✍ Scribed by Kent Carlson; Marion Ehrich

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 407 KB
• Volume: 22
• Category: Article
• ISSN: 1095-6670
• DOI: 10.1002/jbt.20229

✦ Synopsis

The current study investigated the relationship of the cell cycle phase (as G 0 /G 1 , S, and G 2 /M) and cytotoxicity (as sub-G 1 DNA) to determine whether alterations in cell replication were associated with organophosphate (OP) compound induced cytotoxicity. Results demonstrated that, overall, OP compound-induced cell cycle changes were variable and depended on the OP compound, exposure concentration, and temporal relationship to cytotoxicity. Noncytotoxic OP compound treatments substantially decreased the percentage of cells in S phase of the cell cycle when compared to controls. A corresponding increase was seen in the percent of cells in G 0 /G 1 phase of the cell cycle. In the precytotoxic interval of exposure, most cytotoxic OP compound treatments substantially decreased the percentage of cells in G 2 /M phase of the cell cycle. Corresponding increases were seen primarily in G 0 /G 1 phase cells. Effects on cells in S stage of the cell cycle varied with the OP compound. In the during cytotoxic interval of exposure, most cytotoxic OP compound treatments substantially increased the percentage of cells in S phase of the cell cycle. A corresponding decrease in the percent of cells in G 0 /G 1 stage of the cell cycle was observed. Furthermore, treatments either increased or decreased the percentage of cells in G 2 /M phase of the cell cycle when compared to controls, with decreases more likely with the most cy-