Distribution of pyruvate dehydrogenase dihydrolipoamide acetyltransferase (PDC-E2) and another mitochondrial marker in salivary gland and biliary epithelium from patients with primary biliary cirrhosis

✍ Scribed by Ruth E. Joplin; Gerald D. Johnson; John B. Matthews; John Hamburger; J. Gordon Lindsay; Stefan G. Hubscher; Alastair J. Strain; James M. Neuberger

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 790 KB
• Volume: 19
• Category: Article
• ISSN: 0270-9139
• DOI: 10.1002/hep.1840190610

✦ Synopsis

Previous studies in which quantitative immunofluorescenm was used have shown that certain biliary epithelial cells in liver with primary biliary cirrhosis show i n d levels of pyruvate dehydrogenase dihyddipoamide acetyltransferase compared with controls. "his study was designed to determine whether the increase in intensity of pyruvate dehydrogenaee dihydmlipoamide acetyltransferase in biliary epithelial cells is accounted for by an increase in the number of mitochondria in the same cells. A doubleantibody s t u n g technique was used with antibodies specidic for pyruvate dehydrogenase dihydrolipoamide acetyltransferand another mitochondrial inner membrane marker, mcogmwd by the mouse monoclonal antibody MCAlSlA. Distribution of the antigens was studied in sections of liver and salivary gland, an additional site that is frequently involved in primary biliary cirrhosis. Confocal microscopy was used to quantify the intensity of fluorescence resulting from binding of fluomchrome-labeled antibody. In both liver and salivary glands MCA15lA binding was similar in normal and sections with primary biliary cirrhosis and corresponded to the predicted distribution of mitochondria in these h e s . In the liver staining was less intense in biliary epithelial cells than in hepatocytes. In salivary gland binding of both antibodies was predominantly localized to duct cells, with those chondria, being moat intensely stained. There was high coincidencx of the two antigens in salivary glands (p c 0.01) and in biliary epithelial cells from normal liver (p = 0.01). However, in liver with primary biliary cirrhosis, despite high coincidence between the antigens on hepatoeytes, biliary epithelial cells showed f0-W etriated ducts, h o r n to be rich in mito-