Distribution of phosphorylated GAP-43 (neuromodulin) in growth cones directly reflects growth cone behavior
✍ Scribed by Dent, Erik W. ;Meiri, Karina F.
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 510 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0022-3034
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✦ Synopsis
Phosphorylation of GAP-43 (neurowas low in organelle-poor lamellae. However, levels in modulin) by protein kinase C (PKC) occurs at a single lamellae were elevated when they became motile. Consite, serine 41 . In vivo, phosphorylation is induced after versely, levels of phosphorylated GAP-43 were low in initiation of axonogenesis and is confined to distal axons either lamellae that were actively retracting or in the and growth cones. Within individual growth cones, central organelle-rich region and proximal neurite of phosphorylation is nonuniformly distributed. Here, we growth cones that had totally collapsed. The results sughave used high-resolution video-enhanced microscopy gest a mechanism whereby phosphorylation of GAP-43 of cultured dorsal root ganglia neurons together with by PKC, potentially in response to extracellular signals, immunocytochemistry with a monoclonal antibody that could direct the functional behavior of the growth cone. recognizes PKC-phosphorylated GAP-43 to correlate