โ Scribed by Zhong, Cisheng ;Ling, Yiping ;Wu, Zhengquan ;Zhu, Shiya ;Hu, Rongfang
No coin nor oath required. For personal study only.
Intranuclear inclusions have been found in epithelial cells of renal proximal convoluted tubules (RPCT) in lead poisoned animals. It has been suggested previously that the inclusions contained lead. The present report gives evidence to the contrary. Samples of renal cortex were taken from adult rats fed laboratory chow containing 2% lead acetate for 1 month. Under the electron microscope (EM), intranuclear inclusions were observed in epithelial cells of RPCT. They were round or ovoid, and not delineated by a membrane. They consisted of two parts: Peripheral and central. Lysosomes increased in number, and many myelin figures appeared in the cytoplasm. In the lumen of the tubules, high electron-dense concretions were found. By x-ray microranalysis, neither the central part nor the peripheral part of the intranuclear inclusions produced a lead peak in the spectrum. Lysosomes and the cytoplasmic matrix also showed no lead peak. However, when the electron beam was focused on the concretion in the lumen of the RPCT, the L, peak of lead (10.551 keV) and K, peak of calcium (3.691 keV) were evident. From these findings it is suggested that intranuclear inclusions in the cells of RPCT are not lead deposits. Rather, it is the concretions found in the lumen of tubule that contain lead.
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