Distribution of free and membrane-bound ribosomes after corticosterone administration in perfused rat liver

✍ Scribed by T. Ekren

• Publisher: Springer
• Year: 1973
• Tongue: English
• Weight: 276 KB
• Volume: 1
• Category: Article
• ISSN: 0301-4851
• DOI: 10.1007/bf00357157

✦ Synopsis

Contrasting results have been reported on the effect of steroid hormones on the interaction between ribosomes and endoplasmic reticulum in rat liver. Exposure to high gravitational forces for a long time was found necessary to obtain a constant ratio of free to membrane-bound ribosomes from the post-nuclear supernatant. Using these isolation conditions, rat liver from fasted controls and from fasted, adrenalectomized rats contain both about 45% membranebound ribosomes. Addition of corticosterone to the livers from adrenalectomized rats did not increase the pool of bound ribosomes more than in the control livers; 55% was found in both. Corticosterone had therefore no effect on the pool sizes of free and membrane-bound ribosomes in perfused rat livers.