Distribution of endogenous gibberellins in vegetative and reproductive organs ofBrassica

✍ Scribed by Karen P. Zanewich; Stewart B. Rood

• Book ID: 104658623
• Publisher: Springer
• Year: 1993
• Tongue: English
• Weight: 552 KB
• Volume: 12
• Category: Article
• ISSN: 0721-7595
• DOI: 10.1007/bf00193677

✦ Synopsis

Recognizing the physiological diversity of different plant organs, studies were conducted to investigate the distribution of endogenous gibberellins (GAs) in Brassica (canola or oilseed rape). GA 1 and its biosynthetic precursors, GA20 and GA19, were extracted, chromatographically purified, and quantified by gas-chromatography-selected ion monitoring (GC-SIM), using [2H2]GAs as internal standards. In young (vegetative) B. napus cv. Westar plants, GA concentrations were lowest in the roots, increased acropetally along the shoot axis, and were highest in the shoot tips. GA concentrations were high but variable in leaves. GA~ concentrations also increased acropetally along the plant axis in reproductive plants. During early silique filling, GA1 concentrations were highest in siliques and progressively lower in flowers, inflorescence stalks (peduncles plus pedicels), stem, leaves, and roots.