Distribution and peroxidative oxidation of 2-t-butyl-4-methoxyphenol in rat tissues after a single intraperitoneal dose

✍ Scribed by Della Corte, Laura ;Bianchi, Loria ;Valoti, Massimo ;Sgaragli, Giampietro

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 421 KB
• Volume: 4
• Category: Article
• ISSN: 0887-2082
• DOI: 10.1002/jbt.2570040302

✦ Synopsis

The distribution of 2-t-butyl-4-methoxyphenol (BHA) and its conversion to 2,2'-dihydroxy-3,3 '-di-t-butyl-5, 5 '-dimethoxydiphenyl (di-BHA) in rat tissues at different times (1-96 hr) following the intraperitoneal administration of a single dose of BHA (32 mg kg-' body weight) were monitored by gas chromatography-mass spectrometry (GCIMS) analysis of both compounds. High BHA levels were found in the intestine and liver persisting up to 24 hours (5.5-20.7 and 1.8-3.3 pg g-' wet weight, respectively). In these tissues, values of the area under the experimental concentration curve (AUChZ4) were 285 and 49 times higher, respectively, than those observed in plasma (945 ng mL-' hr), AUCSz4 values in kidney, spleen, erythrocytes, and brain were 2-7 times higher, whereas values below those found in plasma were observed in lung and muscle. The metabolite di-BHA could be detected in the intestine, kidney, and spleen, amounting to 5 4 % of BHA. These findings indicate that rat intestine is capable of transforming in vivo BHA into di-BHA even when the former compound is administered intraperitoneally and that this capacity is shared by the kidney and spleen.