Distinct VH repertoires in primary and secondary B cell lymphocyte subsets in the preimmune repertoire of A/J mice: the CRI-A idiotype is preferentially associated with the HSAlow B cell subset
✍ Scribed by Chantal Masungi Luko; Georgette Vansanten; Marion Ryelandt; Olivier Denis; Christian Wuilmart; Fabienne Andris; Annette Van Acker; Maryse Brait; Jean Philippe Cloquet; Naima Ismaili; Françoise Nisol; Dominique Latinne; Alan Brown; Oberdan Leo; Hervé Bazin; Jacques Urbain
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 201 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0014-2980
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✦ Synopsis
The anti-arsonate immune response of A/J mice is characterized by the occurrence of several recurrent idiotypes with a different temporal pattern of expression. The CRI-A idiotype is typically a memory idiotype since it appears late in the primary and dominates the secondary as well as subsequent immune responses. The CRI-C idiotype is present throughout the responses, including the primary one. Naive adult A/J mice treated repeatedly with anti-? or anti-ˇmonoclonal antibodies exhibit a completely different balance of HSA low and HSA high B cell subsets and an opposite idiotype profile after immunization with p-azophenylarsonate coupled to hemocyanin. Anti-? treatment leads to a striking enhancement of the HSA low cell subset associated with an earlier important synthesis of CRI-A + antibodies, while anti-ťreatment enhances significantly the HSA high compartment with a strong decrease of CRI-A and persistence of CRI-C1 antibodies. Semiquantitative PCR analysis reveals that the presence of CRI-A transcripts is associated with the HSA low compartment, while CRI-C transcripts are mainly associated with HSA high B cell subsets. This has been demonstrated with spleen cells of adult A/J mice treated with anti-? or anti-ˇantibodies and also with purified B cell subsets of unimmunized adult A/J mice and on neonatal spleen cells. It appears that the memory (CRI-A) idiotype is selected into the HSA low B cell subset before antigen arrival.