Distance distributions and dynamics of a zinc finger peptide from fluorescence resonance energy transfer measurements

Distance distributions in native and ran

Distance distributions in native and random-coil troponin I from frequency-domain measurements of fluorescence energy transfer

✍ Joseph R. Lakowicz; Ignacy Gryczynski; Herbert C. Cheung; Chien-Kao Wang; Michae 📂 Article 📅 1988 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 523 KB

We used time-dependent fluorescence energy transfer to determine the distribution of donorto-acceptor distances in native and denatured troponin I(Tn1). The single tryptophan residue (Trp 158) of TnI served as the donor (D), and the acceptor (A) was a labeled cysteine residue (Cys 133). The time-dep