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Dissolved oxygen concentration in serum-free continuous culture affects N-linked glycosylation of a monoclonal antibody

✍ Scribed by Jeremy P Kunkel; David C.H Jan; James C Jamieson; Michael Butler


Book ID
119524926
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
243 KB
Volume
62
Category
Article
ISSN
0168-1656

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The murine B-lymphocyte hybridoma, CC9C10 was grown at steady state under serum-free conditions in continuous culture at dissolved oxygen (DO) concentrations in the range of 10% to 150% of air saturation. Cells could be maintained within this range at high viability in a steady state at a dilution r