𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Dissociation of anchorage independence from tumorigenicity in human cell hybrids

✍ Scribed by Eric J. Stanbridge; Joyce Wilkinson

Publisher
John Wiley and Sons
Year
1980
Tongue
French
Weight
796 KB
Volume
26
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The association between anchorage independence and tumorigenicity was examined using a series of intraspecific human cell hybrids. The cell lines represented non‐tumorigenic HeLa/fibroblast hybrids and tumorigenic segregants derived from them. These segregants had lost no more than 5% of the original chromosome complement. Both non‐tumorigenic and tumorigenic cell populations formed colonies in methyl cellulose. The relative size of the colonies seemed to be inherited as a stable trait. Serial cloning of nontumorigenic hybrids in methyl cellulose led to an enhanced efficiency of colony formation but no selection for tumoregenic segregants. Thus, the property of anchorage independence is clearly dissociated from tumorigenicity in this human cell system. An ancillary observation was the chromosomal stability of the hybrids over many population doublings. This intraspecific human cell model therefore provides a genotypically and phenotypically stable system for examination of the genetic control of transformation and neoplasia.

📜 SIMILAR VOLUMES

Alterations in the extracellular matrix
Alterations in the extracellular matrix organization associated with the reexpression of tumorigenicity in human cell hybrids
✍ Channing J. Der; Eric J. Stanbridge 📂 Article 📅 1980 🏛 John Wiley and Sons 🌐 French ⚖ 972 KB

## Abstract The expression of fibronectin on the cell surface was evaluated on a series of intraspecific human cell hybrids formed between HeLa and normal fibroblast strains. Although these hybrids continued to express many of the __in vitro__ transformation properties of their corresponding tumori

Recessive (mediator−) revertants from c-
Recessive (mediator−) revertants from c-h-ras oncogene-transformed NIH 3T3 cells: Tumorigenicity in nude mice and transient anchorage and serum independence of the recovered tumor cells in culture
✍ Toshiko Omata-Yamada; Hisafumi Yamada; Peter Lengyel 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 945 KB

## Abstract We have reported earlier the isolation of two recessive, serum‐ and anchoragedependent revertants (R116 and R260) from a c‐H‐ras oncogene‐transformed NIH 3T3 line. In both revertants, the oncogene was fully expressed and fusion of either revertant with (untransformed) NIH 3T3 cells, or

Deletion of the PDZ motif of HPV16 E6 pr
Deletion of the PDZ motif of HPV16 E6 preventing immortalization and anchorage-independent growth in human tonsil epithelial cells
✍ William C. Spanos; Jeremy Geiger; Mary E. Anderson; George F. Harris; Aaron D. B 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 307 KB

## Abstract ## Background Human papillomavirus 16 (HPV16) has been associated with head and neck squamous cell carcinoma (HNSCC) in up to 60% of sampled specimens. ## Methods To understand better the viral genes required to transform human tonsil epithelial cells (HTEC), we isolated HTEC's and t

Enhancement of anchorage-independent gro
Enhancement of anchorage-independent growth of human pancreatic carcinoma MIA PaCa-2 cells by signaling from protein kinase C to mitogen-activated protein kinase
✍ Keiko Ishino; Hidesuke Fukazawa; Mayumi Shikano; Motoi Ohba; Toshio Kuroki; Yosh 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 222 KB

## Abstract We found that 12‐__O__‐tetradecanoylphorbol‐13‐acetate (TPA) promoted anchorage‐independent growth but did not affect anchorage‐dependent growth of MIA PaCa‐2 human pancreatic carcinoma cells. TPA markedly activated mitogen‐activated protein kinase (MAPK)/extracellular signal–regulated