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Dissociation of aminoacyl tRNA from the complex of EF-Tu--GTP--aminoacyl tRNA by extracts of Escherichia coli

✍ Scribed by Anthony V. Furano


Book ID
118311588
Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
497 KB
Volume
76
Category
Article
ISSN
0006-291X

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Purification of Aminoacyl-tRNA by Affini
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Elongation factor Tu from Thermus thermophilus containing six histidine residues on its C-terminus, EF-Tu( \(\left.\mathrm{CHis}_{6}\right)\), was used for purification of aminoacyltRNA isoacceptors, from aminoacylated bulk tRNA, by affinity chromatography. Preformed aminoacyltRNA.EF-Tu \(\left(\mat