Dissociation Kinetics of the Streptavidin–Biotin Interaction Measured Using Direct Electrospray Ionization Mass Spectrometry Analysis

The use of a stable internal mass standa

The use of a stable internal mass standard for accurate mass measurements of oligonucleotide fragment ions using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry with infrared multiphoton dissociation

✍ Steven A. Hofstadler; Richard H. Griffey; Ljiljana Pasa-Tolic; Richard D. Smith 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 85 KB 👁 2 views

In this work we demonstrate the use of peptide as a stable internal mass standard to obtain accurate mass measurements of infrared multiphoton dissociation (IRMPD) generated phosphorothioate oligonucleotide fragment ions. Because oligonucleotides are more readily dissociated in the gas phase by IRMP