Dissection of the Ability of the Chicken Lysozyme Gene 5‘ Matrix Attachment Region To Stimulate Transgene Expression and To Dampen Position Effects †,‡

Use of the chicken lysozyme 5′ matrix at

Use of the chicken lysozyme 5′ matrix attachment region to generate high producer CHO cell lines

✍ Pierre-Alain Girod; Monique Zahn-Zabal; Nicolas Mermod 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 332 KB

Scaffold or matrix attachment region (S/MAR) genetic elements have previously been proposed to insulate transgenes from repressive effects linked to their site of integration within the host cell genome. We have evaluated their use in various stable transfection settings to increase the production o