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Disruption of the β-sheet structure of a protected pentapeptide, related to the β-amyloid sequence 17–21, induced by a single, helicogenic Cα-tetrasubstituted α-amino acid

✍ Scribed by Dr Fernando Formaggio; Andrea Bettio; Vittorio Moretto; Marco Crisma; Claudio Toniolo; Quirinus B. Broxterman


Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
95 KB
Volume
9
Category
Article
ISSN
1075-2617

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✦ Synopsis


Abstract

Fifteen years ago it was shown that an α‐aminoisobutyric acid (Aib) residue is significantly more effective than an L‐Pro or a D‐amino acid residue in inducing β‐sheet disruption in short model peptides. As this secondary structure element is known to play a crucial role in the neuropathology of Alzheimer's disease, it was decided to check the effect of Aib (and other selected, helix inducer, C^α^‐tetrasubstituted α‐amino acids) on the β‐sheet conformation adopted by a protected pentapeptide related to the sequence 17–21 of the β‐amyloid peptide. By use of FT‐IR absorption and ^1^H NMR techniques it was found that the strong self‐association characterizing the pentapeptide molecules in weakly polar organic solvents is completely abolished by replacing a single residue with Aib or one of its congeners. Copyright © 2003 European Peptide Society and John Wiley & Sons, Ltd.