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Disruption of the peroxisomal citrate synthase CshA affects cell growth and multicellular development in Dictyostelium discoideum

โœ Scribed by Ying-Chieh Huang; Yi-Hsing Chen; San-Ren Lo; Chia-I Liu; Cheng-Wei Wang; Wen-Tsan Chang


Book ID
110832052
Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
630 KB
Volume
53
Category
Article
ISSN
0950-382X

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โœ Bain, Gerard ;Tsang, Adrian ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› Springer ๐ŸŒ English โš– 881 KB

We have used homologous recombination to disrupt the gene which codes for p34 and p31, two polypeptides related to a cAMP-binding protein (CABP1) in Dictyostelium discoideum. By screening a total of 80 independent transformants by Southern blotting, four mutants have been isolated. Two of these muta