We describe here the construction of six deletion mutants and their basic phenotypic analysis. Six open reading frames (ORFs) from chromosome X, YJR039w, YJR041c, YJR043c, YJR046w, YJR053w and YJR065c, were disrupted by deletion cassettes with long (LFH) or short (SFH) flanking regions homologous to
✦ LIBER ✦
Disruption of six novel yeast genes located on chromosome II reveals one gene essential for vegetative growth and two required for sporulation and conferring hypersensitivity to various chemicals
✍ Scribed by R. Kucharczyk; R. Gromadka; A. Migdalski; P. P. Słonimski; J. Rytka
- Book ID
- 111697397
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 495 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0749-503X
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Deletion via PCR-mediated gene replacement, together with basic functional and bioinformatic analyses, have been performed on six novel open reading-frames (ORFs) on the left arm of chromosome XII of Saccharomyces cerevisiae (YLL033w, YLL032c, YLL031c, YLL030c, YLL029w and YLL028w). ORF deletion was