Disposable Glucose Sensors for Flow Injection Analysis Using Substituted 1,4-Benzoquinone Mediators
✍ Scribed by King-Tong Lau; Stephane A. L. de Fortescu; Lindy J. Murphy; Jonathan M. Slater
- Publisher
- John Wiley and Sons
- Year
- 2003
- Tongue
- English
- Weight
- 150 KB
- Volume
- 15
- Category
- Article
- ISSN
- 1040-0397
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✦ Synopsis
Abstract
The redox chemistry of several substituted benzoquinones was investigated by cyclic voltammetry at a glassy carbon electrode and candidates for inclusion in a mediated biosensor for use in flow analysis were selected on the basis of oxidation potential, electrochemical reversibility and solubility. Glucose sensors constructed by sequential deposition onto a carbon pellet of 2,6‐dimethyl‐1,4‐benzoquinone, 2,3,5,6‐tetramethyl‐1,4‐benzoquinone or phenyl‐1,4‐benzoquinone mediator solution, followed by glucose oxidase in polyvinylalcohol‐Nafion solution, were tested for response to glucose using flow injection analysis. Sensors prepared from 2,6‐dimethyl‐1,4‐benzoquinone gave highest sensitivity, with a linear range of response to glucose of 2.5–40 mM. The use of an enzyme‐free comparative electrode to eliminate the response from interferents was investigated.