Disposable Electrodes for Capacitive Immunosensor

✍ Scribed by Kosin Teeparuksapun; Proespichaya Kanatharana; Warakorn Limbut; Chongdee Thammakhet; Punnee Asawatreratanakul; Bo Mattiasson; Booncharoen Wongkittisuksa; Chusak Limsakul; Panote Thavarungkul

• Book ID: 102831033
• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 511 KB
• Volume: 21
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/elan.200804517

✦ Synopsis

Abstract

Disposable electrodes were fabricated by coating chromium (5 nm) and gold (200 nm) on glass strips (5.0 mm×25.4 mm) and used in a label‐free immunosensor. Human serum albumin (HSA) and its antigen (anti‐HSA) were used as a model system. Electropolymerization of o‐phenylenediamine was used for the immobilization of anti‐HSA by covalent binding. A linear relationship was obtained in the range from 1.0×10^−14^ to 1.0×10^−9^ M with a limit of detection of 8.0×10^−15^ M. Each modified electrode can be reused up to 30 times. The developed system was applied for human serum samples and compared to Albumin BCG method.