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Discrimination of G1, S, and G2 cells using high-resolution TV-scanning and multivariate analysis methods

✍ Scribed by W. Abmayr; W. Giaretti; P. Gais; P. Dörmer


Book ID
102814783
Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
735 KB
Volume
2
Category
Article
ISSN
0196-4763

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✦ Synopsis


Images of exponentially growing mouse L fibroblasts were used to test whether GI, S, and Gz cells could be discriminated by means of densitometric, geometric, textural and chromatin features. The cells were preincubated with FUdR and labeled with 3H-TdR. The definition of GI, S, and Gz was based on autoradiography used to define labeled S

Chromatin features


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Flow cytometric analysis of G1- and G2/M
✍ Michael Nüsse; Wolfgang Beisker; Christine Hoffmann; Attila Tarnok 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 790 KB

## Abstract Several subcompartments of the cell cycle in addition to the G~1~‐, S‐, and G~2~‐phases usually observed were identified by simultaneous flow cytometric measurements of ethidium bromide fluorescence and side scatter intensity of cell nuclei. Metaphase cells and very early G~1~‐phase cel