Discrimination of G1, S, and G2 cells using high-resolution TV-scanning and multivariate analysis methods
✍ Scribed by W. Abmayr; W. Giaretti; P. Gais; P. Dörmer
- Book ID
- 102814783
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 735 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0196-4763
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✦ Synopsis
Images of exponentially growing mouse L fibroblasts were used to test whether GI, S, and Gz cells could be discriminated by means of densitometric, geometric, textural and chromatin features. The cells were preincubated with FUdR and labeled with 3H-TdR. The definition of GI, S, and Gz was based on autoradiography used to define labeled S
Chromatin features
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## Abstract Several subcompartments of the cell cycle in addition to the G~1~‐, S‐, and G~2~‐phases usually observed were identified by simultaneous flow cytometric measurements of ethidium bromide fluorescence and side scatter intensity of cell nuclei. Metaphase cells and very early G~1~‐phase cel