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Discrimination between mammalian RNases H-1 and H-2

✍ Scribed by Mehran Goulian; Cheryl J. Heard


Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
505 KB
Volume
192
Category
Article
ISSN
0003-2697

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✦ Synopsis


The two principal RNases H in mammalian cells, H-1 and H-2, differ in their responses to sale, divalent metal, and sulfhydryl inhibition. Specific reaction conditions that provide unambiguous discrimination between RNases H-1 and H-2 with only two assays are described. The assays were used for identification in a new purification procedure for RNases H-1 and H-2.


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