Discrimination between mammalian RNases H-1 and H-2
β Scribed by Mehran Goulian; Cheryl J. Heard
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 505 KB
- Volume
- 192
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
β¦ Synopsis
The two principal RNases H in mammalian cells, H-1 and H-2, differ in their responses to sale, divalent metal, and sulfhydryl inhibition. Specific reaction conditions that provide unambiguous discrimination between RNases H-1 and H-2 with only two assays are described. The assays were used for identification in a new purification procedure for RNases H-1 and H-2.
π SIMILAR VOLUMES
Cellular low-level luminescence was measured after various disintegrative processes in brain cell preparations. In addition t o known origins of low-level luminescence, e.g. oxygen radical reactions or enzymatic and non-enzymatic redox systems, a further source of photon emission is reported which i