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Discovering innate immunity genes using differential display: A story of RNA helicases

✍ Scribed by Zaida G. Ramirez-Ortiz; Rajas V. Warke; Laura Pacheco; Kris Xhaja; Devanand Sarkar; Paul B. Fisher; Sunil K. Shaw; Katherine J. Martin; Irene Bosch


Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
423 KB
Volume
209
Category
Article
ISSN
0021-9541

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✦ Synopsis


Abstract

In this review we discuss the current literature for RNA helicases in response to RNA virus infection. We show the use of Differential Display Reverse Transcription PCR methodology (DD) to analyze virus–host interactions and we present current findings in dengue virus‐induced gene expression of RNA helicases. J. Cell. Physiol. 209: 636–644, 2006. © 2006 Wiley‐Liss, Inc.


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Refinements of the Differential Display
✍ Rama Rao Amara; Vijaya Satchidanandam 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 100 KB

Differential display reverse transcription PCR (DDRT-PCR) 2 is a popular technique used to identify differences in messenger RNA species expressed under different conditions (1-4). The use of this technique in prokaryotic systems has been greatly hampered by the lack of a poly(A) tail at the 3Ј end